Cell-Specific Regulation of Inflammatory Cytokines and Acute-Phase Proteins by the Glucocorticoid Receptor

Figure 8

Dual luciferase assays of chemokine promoter activities in HEPG2 and HEK293T cell lines. HEPG2 (a–d) and HEK293T (e and f) cells were transfected with luciferase reporter plasmids for promoters of CXCL1 (a and e), CXCL2 (b and f), CXCL8 (c and g), and CXCL10 (d and h) and GR expression plasmid in dexamethasone-treated groups. Cells were incubated with plasmids overnight and then treated with dexamethasone 10 nM, TNFα 20 ng/mL, and/or DMSO (0.1%) control. Promoter activities were quantified by dual-luciferase assay 24 hr after drug treatment. Mean ± SE, n = 3–4-well replicates, and , , and . ns, not significantly different.

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