PPAR-γ Activation Alleviates Osteoarthritis through Both the Nrf2/NLRP3 and PGC-1α/Δψ_m Pathways by Inhibiting Pyroptosis

Figure 1

The upregulated levels of NLRP3, caspase-1 P10, and GSDMD-N in the cartilage of the lateral femoral condyle of the knee of a patient with severe OA. (a and b) MRI shows the lateral femoral condyle of the human knee, which has mild and severe lesions. MRI scoring was used to assess injury severity. (c–h) The levels of caspase-1 P10, GSDMD-N, and NLRP3 in mild and severe knee osteoarthritis are shown using immunohistochemical staining (bar: 200 μm). Quantification of the immunohistochemical staining is located below. (i and j) Representative immunoblots of NLRP3, caspase-1 P10, and GSDMD-N in the mild and severe lesion groups. GAPDH was used as an internal reference. Densitometric analysis of the immunoblots is located on the right. The data represent three independent experiments. * and **.