Research Article

PPAR-γ Activation Alleviates Osteoarthritis through Both the Nrf2/NLRP3 and PGC-1α/Δψm Pathways by Inhibiting Pyroptosis

Figure 6

Involvement of Nrf2 and PGC-1α in the effect of Piog on pyroptosis in the OA chondrocytes. (a and b) Western blotting experiments and densitometric analysis. SR was used as a PGC-1α inhibitor. Nrf2-siRNA was used for knocking down the level of Nrf2. GAPDH was used as an internal reference. (c) ELISA was used to measure IL-1β and IL-18 concentrations. (d) CCK8 assays were performed to measure chondrocyte viability. **, . (e) The action mode of PPAR-γ under Piog stimulation to protect from pyroptosis-mediated OA cartilage damage.
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