PPAR Research / 2023 / Article / Tab 2 / Research Article
PPARβ /δ Agonism with GW501516 Increases Myotube PGC-1α Content and Reduces BCAA Media Content Independent of Changes in BCAA Catabolic Enzyme Expression Table 2 Summary of qRT-PCR primers from Integrated DNA Technologies (Coralville, IA).
Gene abbreviation Forward sequence Reverse sequence Atp5b 5 - AGGCCCTTTGCCAAGCTT-3 5 - TTCTCCTTAGATGCAGCAGAGTACA-3 Bcat2 5 - CGGACCCTTCATTCGTCAGA-3 5 - CCATAGTTCCCCCCCAACTT-3 Bckdha 5 - CCAGGGTTGGTGGGATGAG-3 5 - GGCTTCCATGACCTTCTTTCG-3 Cebp 5 - GTGTGCACGTCTATGCTAAACCA-3 5 - GCCGTTAGTGAAGAGTCTCAGTTTG-3 Cox5a 5 - GCTGCATCTGTGAAGAGGACAAC-3 5 - CAGCTTGTAATGGGTTCCACAGT-3 Cs 5 - TGAGAGGCATGAAGGGACTTGTGT-3 5 - ATCTGTCCAGTTACCAGCAGCCAA-3 Fat (CD36) 5 - TAGTAGAACCGGGCCACGTA-3 5 - CAGTTCCGATCACAGCCCAT-3 Slc2a4 (Glut4) 5 - GATGAGAAACGGAAGTTGGAGAGA-3 5 - GCACCACTGCGATGATCAGA-3 Hibadh 5 - GCAGCGGTGTGTTCTAGGTC-3 5 - ACACGTCATAGAGGATGAGTGG-3 Ldha 5 - GGCTTGTGCCATCAGTATCT-3 5 - CCCGCCTAAGGTTCTTCATTAT-3 Ldhb 5 - AGTCTCCCGTGCATCCTCAA-3 5 - AGGGTGTCCGCACTCTTCCT-3 Nrf1 5 - ACCCTCAGTCTCACGACTAT-3 5 - GAACACTCCTCAGACCCTTAAC-3 Pdh 5 - GAAGGCCCTGCATTCAACTTC-3 5 - ATAGGGACATCAGCACCAGTGA-3 Ppargc1a 5 - GACAATCCCGAAGACACTACAG-3 5 - AGAGAGGAGAGAGAGAGAGAGA-3 Ppara 5 - CTCGCGTGTGATAAAGC-3 5 - CGATGCTGTCCTCCTTG-3 Ppard 5 - GCCTCGGGCTTCCACTAC-3 5 - AGATCCGATCGCACTTCTCA-3 Pparg 5 - TTCAGCTCTGGGATGACCTT-3 5 - CGAAGTTGGTGGGCCAGAAT-3 Scd1 5 - CATCGCCTGCTCTACCCTTT-3 5 - GAACTGCGCTTGGAAACCTG-3 Srebp1 5 - ATCGCAAACAAGCTGACCTG-3 5 - AGATCCAGGTTTGAGGTGGG-3 Tbp 5 - GGGATTCAGGAAGACCACATA-3 5 - CCTCACCAACTGTACCATCAG-3 Tfam 5 - GAAGGGAATGGGAAAGGTAGAG-3 5 - ACAGGACATGGAAAGCAGATTA-3
Abbreviations: Atp5b : ATP synthase F1; Bcat2 : branched-chain aminotransferase 2, Bckdha : branched-chain alpha-keto acid dehydrogenase; Cebpa : CCAAT/enhancer-binding protein alpha; Cox5a : cytochrome C oxidase Subunit 5A; Cs : citrate synthase; Fat or CD36 : fatty acid translocase; Slc2a4 or Glut4 : glucose transporter 4; Hibadh : 3-hydroxyisobutyrate dehydrogenase; Ldha : lactate dehydrogenase a; Ldhb : lactate dehydrogenase b; Nrf1 : nuclear respiratory factor 1; Pdh : pyruvate dehydrogenase; Ppargc1a : peroxisome proliferator-activated receptor gamma coactivator 1-alpha; Pk1 : pyruvate kinase 1; Ppara : peroxisome proliferator-activated receptor alpha; Ppard : peroxisome proliferator-activated receptor delta; Pparg : peroxisome proliferator-activated receptor gamma; Scd1 : stearoyl-CoA desaturase; Srebp1 : sterol regulatory element-binding protein; Tbp : TATA box binding protein; and Tfam : mitochondrial transcription factor A.