Appraisal of the Possible Role of PPARγ Upregulation by CLA of Probiotic Pediococcus pentosaceus GS4 in Colon Cancer Mitigation

Figure 8

Probiotic P. pentosaceus GS4 induced apoptosis in human colon cancer cells: (a) The morphological assessment of apoptosis: The HCT-116 cells were pre-incubated for 1 hour with/without BADGE (20 μM) before treatment with CLA/CLA_GS4 for 48 hour. Following incubation, the apoptosis was determined at morphological level by staining the cells with dual stain (AO/EtBr) and subsequently images were observed under fluorescence microscope and images were captured (scale 100 μm). FACS based assessment of apoptosis: (b) Following scheduled treatment for 48 hours, the HCT-116 cells were analysed by annexin V/PI staining subsequently FACS analysis. (b) The histogram of percentage of apoptosis at different stage. (c) The graphical representation of % apoptotic cells (apoptotic index: AI, annexin V positive cells) among different groups. Statistical significance was indicated as *versus control; #versus BADGE control.

(a)

(b)

(c)