Fibroblast Activation Protein Expression in Sarcomas

Figure 1

Representative H&E and FAP IHC images. In panels a–j, top images show low and high power views of H&E slide and bottom images show low and high power views of FAP stained slide. In panel (k), top left shows low power view of H&E slide, top middle shows high power view of H&E slide, bottom left shows low power view of FAP stained slide, bottom middle shows high power view of FAP stained slide, and top and bottom right shows high power view of FAP stained slide with arrows pointing to blood vessels. In panel (l), top image shows low power view of FAP stained slide and bottom image shows high power view of FAP stained slide. Scale bars are shown for each image. (a) A case of ASPS (FAP.ASPS.2.primary) with FAP staining in both tumor and stromal cells; (b) normal tissue adjacent to the ASPS case shown in panel A (FAP.ASPS.2.normal) with low intensity FAP staining in some stromal cells; (c) a case of ASPS (FAP.ASPS.3.primary) with FAP staining most notable in stromal cells; (d) a case of DF (FAP.DF.3.primary) with intense and dense FAP staining; (e) normal adjacent tissue to the DF case shown in panel D without FAP staining; (f) a case of myxofibrosarcoma (FAP.MFS.4.primary.1) with intense and dense FAP staining seen in the majority of the sample; (g) SFT case (FAP.SFT.5.primary) with intense and dense FAP staining seen in the majority of the cells; (h) a case of SS (FAP.SS.1.recurrence) without FAP staining; (i) a case of UPS (FAP.UPS.4.primary) with intense and dense FAP staining seen in the majority of the sample; (j) a case of UPS (FAP.UPS.1.primary) with dense FAP staining seen in the majority of the sample with higher intensity in stromal cells compared to tumor cells; (k) a case of osteosarcoma (FAP.OS.4.primary.2) with FAP staining that is most notable in stromal cells sections around blood vessels; and (l) colon cancer positive control.

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