Genomic Breakpoint Characterization and Transcriptome Analysis of Metastatic, Recurrent Desmoplastic Small Round Cell Tumor

Figure 4

The EWSR1-WT1 signature in recurrent DSRCT: (a) heatmap of the EWSR1-WT1-regulated gene set identified by Gedminas et al. Log2FC of DSRCT versus LIHC normal tissues, DSRCT versus SARC normal tissues, recurrent versus primary DSRCTs, and siCtrl versus siEWSR1-WT1 in JN-DSRCT-1, and BER-DSRCT are plotted. (b) GSEA of DSRCT versus SARC normal tissues and DSRCT versus LIHC normal tissues on EWSR1-WT1 upregulated and downregulated gene sets. (c) GSEA of recurrent versus primary DSRCT log2FC on EWSR1-WT1 upregulated and downregulated gene sets. (d) RT-qPCR gene expression analysis of EWSR1-WT1 (via WT1 targeting primers) plus four EWSR1-WT1 upregulated genes and four EWSR1-WT1 downregulated genes in primary versus recurrent DSRCTs (n = 2,  < 0.05,  < 0.01, and  < 0.001).

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