Directing Parthenogenetic Stem Cells Differentiate into Adipocytes for Engineering Injectable Adipose Tissue

Figure 5

Chondrogenic differentiation potential of eMSCs and BMSCs. (a) RT-PCR analysis of the gene expression profiles related to chondrogenic differentiation. RNA samples from uninduced cells (UN) and induction cells (days +). (b) Semiquantitative analysis of gene expression levels by RT-PCR. RNA samples from uninduced cells (UN) and induction cells (days +). The relative expression of each gene was compared with the expression of β-actin in the same sample. Values are the means ± SD from 3 independent experiments. , . (c) Micrograph of cells 21 d after chondrogenic induction. Safranin O staining (red) for negatively charged proteoglycans 21 d after chondrogenic induction or uninduced cells (UN). Bars = 100 μm, (d) immunofluorescent staining for aggrecan and Col2a1, antibody staining (green), with DAPI nuclear staining (blue).

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