Allogenic Adipose Tissue-Derived Mesenchymal Stem Cells Ameliorate Acute Hepatic Injury in Dogs

Table 3

Primers used for real-time quantitative RT-PCR.

Gene		Sequence (5-3)	Length (bp)	Accession number
TNFα	For	ACCACACTCTTCTGCCTGCT	259	DQ923808
	Rev	GATAGTGCCGTCAGATGGGT
IL-1β	For	ATGAGGGCATCCAGTTGCA	62	NM_001037971
	Rev	CAAGAGTCTGAGGCATTTCGTG
IL-6	For	CTCTGCACTGAGAAAGGAGATG	132	NM_001003301
	Rev	CTTCCAATCTGGGTTCAATCA
IL-8	For	TTGCCTTGGTCTCTTCTTTATTCC	67	NM_001003200
	Rev	AGCAAGCATCCTACCTCACAGAA
IFNγ	For	GAAAAGGAGTCAGAATCTGTTTCGA	63	NM_001003174
	Rev	TAATGGTCATCCTGCCTGCA
IL-4	For	TAGCACTCACCAGCACCTTTGT	119	AF239917
	Rev	TGCATGGAGCTGACTGTCAAG
IL-10	For	ACTTTGATGACGATGAGCTATGGA	73	XM_014109407
	Rev	CCGTCGGCATTCCCATACT
HGF	For	AAAGGAGATGAGAAACGCAAACAG	95	NM_001002964
	Rev	GGTATTACTGAAGCTTGCTAGGCC
VEGFA	For	TCAGGACACTGCTGTACTTTGAGG	133	NM_001003175
	Rev	GGCTTGTCAGGAGCAAGTGAA
GAPDH^a	For	GATGGGCGTGAACCATGAG	131	NM_001003142
	Rev	TCATGAGGCCCTCCACGAT
GUS^a	For	CCTCCTGCCGTATTACCCTTG	117	NM_001003191
	Rev	TCTGGACGAAGTAACCCTTGG
RPS5^a	For	TCACTGGTGAGAACCCCCT	141	XM_533568
	Rev	CCTGATTCACACGGCGTAG

^aReference gene. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was identified as the most stable reference gene for normalization of relative mRNA concentration using the basic GeNorm visual application for Microsoft Excel (https://genorm.cmgg.be/).