Stem Cells International / 2017 / Article / Tab 3 / Research Article
Allogenic Adipose Tissue-Derived Mesenchymal Stem Cells Ameliorate Acute Hepatic Injury in Dogs Table 3 Primers used for real-time quantitative RT-PCR.
Gene Sequence (5 -3 ) Length (bp) Accession number TNFα For ACCACACTCTTCTGCCTGCT 259 DQ923808 Rev GATAGTGCCGTCAGATGGGT IL-1β For ATGAGGGCATCCAGTTGCA 62 NM_001037971 Rev CAAGAGTCTGAGGCATTTCGTG IL-6 For CTCTGCACTGAGAAAGGAGATG 132 NM_001003301 Rev CTTCCAATCTGGGTTCAATCA IL-8 For TTGCCTTGGTCTCTTCTTTATTCC 67 NM_001003200 Rev AGCAAGCATCCTACCTCACAGAA IFNγ For GAAAAGGAGTCAGAATCTGTTTCGA 63 NM_001003174 Rev TAATGGTCATCCTGCCTGCA IL-4 For TAGCACTCACCAGCACCTTTGT 119 AF239917 Rev TGCATGGAGCTGACTGTCAAG IL-10 For ACTTTGATGACGATGAGCTATGGA 73 XM_014109407 Rev CCGTCGGCATTCCCATACT HGF For AAAGGAGATGAGAAACGCAAACAG 95 NM_001002964 Rev GGTATTACTGAAGCTTGCTAGGCC VEGFA For TCAGGACACTGCTGTACTTTGAGG 133 NM_001003175 Rev GGCTTGTCAGGAGCAAGTGAA GAPDHa For GATGGGCGTGAACCATGAG 131 NM_001003142 Rev TCATGAGGCCCTCCACGAT GUSa For CCTCCTGCCGTATTACCCTTG 117 NM_001003191 Rev TCTGGACGAAGTAACCCTTGG RPS5a For TCACTGGTGAGAACCCCCT 141 XM_533568 Rev CCTGATTCACACGGCGTAG
a Reference gene. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was identified as the most stable reference gene for normalization of relative mRNA concentration using the basic GeNorm visual application for Microsoft Excel (https://genorm.cmgg.be/ ).