Successful Low-Cost Scaffold-Free Cartilage Tissue Engineering Using Human Cartilage Progenitor Cell Spheroids Formed by Micromolded Nonadhesive Hydrogel

Figure 2

The CPC spheroids formed in the micromolded nonadhesive hydrogel with 800 μm diameter in each circular recesses showed stability in their diameter along 21 days of cell spheroid culture. (a, c) Representative images of CPC spheroids formed in micromolded nonadhesive hydrogel with 300 μm diameter (a) and with 800 μm diameter in each circular recesses (c). Phase contrast microscopy. Bar size: 100 μm. (b, d) The graphs represent the mean ± standard error of the spheroid diameter in micromolded nonadhesive hydrogel with 300 μm diameter (b) and with 800 μm diameter in each circular recesses (d). Light gray bar represents 2 days of cell spheroid culture. Dark gray bar represents 21 days of cell spheroid culture. ; .

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