Successful Low-Cost Scaffold-Free Cartilage Tissue Engineering Using Human Cartilage Progenitor Cell Spheroids Formed by Micromolded Nonadhesive Hydrogel

<div>The CPC spheroids formed in the hydrogel with 800 <i>μ</i>m diameter in each circular recesses show enhanced resistance to compression after spontaneous chondrogenesis (21-day culture) compared to those cultured for 2 days (spheroids compaction). (a) Equipment used to perform the loading tests. (b, c) One CPC spheroid between 2 plates before and after compression, respectively. Note spheroid deformation of 25% of the original diameter in (c). Five compression cycles were performed, with a load phase duration of 20 seconds and a recovery phase of 10 seconds. (d) Data collected from 3 spheroids of three different samples after spheroid compaction (2 days, light gray column) and after spontaneous chondrogenesis (21 days, dark gray column) are expressed as mean ± SD. <span class="nowrap"><svg height="13.532pt" id="M17" style="vertical-align:-3.4295pt" version="1.1" viewbox="-0.0498162 -10.1025 79.8748 13.532" width="79.8748pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 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Stem Cells International

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Figure 5

Figure 5: Successful Low-Cost Scaffold-Free Cartilage Tissue Engineering Using Human Cartilage Progenitor Cell Spheroids Formed by Micromolded Nonadhesive Hydrogel 

Figure 5 | Successful Low-Cost Scaffold-Free Cartilage Tissue Engineering Using Human Cartilage Progenitor Cell Spheroids Formed by Micromolded Nonadhesive Hydrogel 