Gene Profiles in the Early Stage of Neuronal Differentiation of Mouse Bone Marrow Stromal Cells Induced by Basic Fibroblast Growth Factor

Figure 6

Overexpression of Mash-1 promotes neuronal differentiation of BMSCs. (a) RT-PCR (left) and western blot (right) representation of selected markers from cDNA microarray which showed consistencies with the data as analyzed. (b) Distribution of neuronal differentiation markers. Immunofluorescence staining using specific antibodies against GAP43, Mash-1, and Hes5 was performed in BMSCs with bFGF treatment for 24 hours. NC: negative control. Images are representatives of at least three experiments. (c) Mash-1 overexpressing BMSCs showed a typical neuronal morphology. (d) Western blotting analysis showed a significant increase of the NSE expression in Mash-1 overexpressing BMSCs compared to control cells. (e) The expression of SV2a and SNAP-25 were determined by qRT-PCR in Mash-1 overexpressing BMSCs. Data of RT-PCR indicate the of three experiments.

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