Kartogenin Improves Osteogenesis of Bone Marrow Mesenchymal Stem Cells via Autophagy

Figure 1

KGN enhances the osteogenic ability of BMMSCs without affecting cell proliferation. Cell proliferation capacity of BMMSCs was measured by CCK-8 (a), immunofluorescent staining (b) of Ki67, and semiquantification (c) of (b) between the control and KGN-treated BMMSCs. , independent Student’s test. ARS staining detection (d) and semiquantification (e) of mineralized nodules. KGN promoted osteogenesis of BMMSCs. Scale  μm. , independent Student’s test. Western blotting (f) and semiquantification analysis (g, h) and qRT-PCR (i, j) of osteogenic markers ALP and Runx2. KGN upregulated the expression of osteogenic genes. and , independent Student’s test.

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