PPARβ/δ-Interfering Peptide Enhanced Mesenchymal Stromal Cell Immunoregulatory Properties

Figure 3

Expression profile of PPARβ/δ, PPARα, and PPARγ target genes in hAD-MSC inactivated for PPARβ/δ. (a, b) Comparison of the expression levels of PPARβ/δ target genes (PDK4 and ANGPTL4) between untreated (UT) hAD-MSC (dashed line), GSK3787-pretreated hAD-MSC (GSK), and PP11-pretreated hAD-MSC (PP11). PDK4 (a) and ANGPTL4 (b) expressions were normalized to housekeeping gene (RPS9) expression. mRNA expression levels in hAD-MSC treated during 24 h with 0.1 μM of GSK3787 (GSK) or 0.1 μM of PP11 were standardized to expression in nonpretreated hAD-MSC (dashed line), and results are expressed using the method. (c, d) Comparison of the expression levels of PPARα target genes (ACOX1 and CPT1A) between untreated hAD-MSC (dashed line), GSK3787-pretreated hAD-MSC (GSK), and PP11-pretreated AD-MSC (PP11). ACOX1 (c) and CPT1A (d) expressions were normalized to housekeeping gene (RPS9) expression. mRNA expression levels in hAD-MSC treated during 24 h with 0.1 μM of GSK3787 (GSK) or 0.1 μM of PP11 were standardized to expression in untreated hAD-MSC (dashed line), and results are expressed using the method. (e) Comparison of the expression level of PPARγ target gene (FABP4) between untreated hAD-MSC (dashed line), GSK3787-pretreated hAD-MSC (GSK), and PP11-pretreated hAD-MSC (PP11). FABP4 expression was normalized to housekeeping gene (RPS9) expression. mRNA expression levels in hAD-MSC treated during 24 h with GSK3787 (GSK, 0.1 μM) or PP11 (0.1 μM) were standardized to expression in untreated hAD-MSC (dashed line), and results are expressed using the method. These results were obtained with hAD-MSC from 4 donors () in 2 or 3 independent experiments. The statistical analysis was performed using the Mann–Whitney test. Statistical significance was noted as ns for , for , for , and for .

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