Research Article
Electrophysiological Changes of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes during Acute Hypoxia and Reoxygenation
Figure 6
The effect of hypoxia and reoxygenation on hiPSC-CM morphology and HIF1-α expression. (a) Differences in the hiPSC-CM sarcomere structure (cMyBP-C, green) were observed after hypoxia and reoxygenation (scale bar 50 μm), and the nucleus area was observed to decrease (counterstained with DAPI, blue). The changes in the HIF1-α (magenta) expression were not obvious via ICC. Confocal images of the cTnT staining of the control, hypoxia, and hypoxia-reoxygenation samples are presented in Supplementary Figure S3. (b) Phase-contrast image of the hiPSC-CMs seeded on MEA before and after hypoxia-reoxygenation treatment showed changes in the CM morphology after hypoxia-reoxygenation compared to before (scale bar 500 μm). (c) Area of the hiPSC-CM nuclei was observed to decrease after hypoxia (H) and reoxygenation (HR) compared to controls (CH and CHR) (d) Expression of HIF1-α from western blot was observed to decrease after hypoxia and hypoxia-reoxygenation compared to control. The blot presents two biological replicates for each condition. (e) Example blots of β-actin and HIF1-α from western blot. Full blots are presented in Supplementary Figure S4. and .
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