Human Umbilical Cord Perivascular Cells Prevent Tumor Growth in a Melanoma Tumor-Bearing Mouse Model and Modulate Breast Cancer and Melanoma Cells in a Cell Line-Dependent Manner In Vitro

Figure 3

Analysis of cell proliferation, apoptosis, and vascularization in SK-MEL-28 tumors of xenograft mouse model treated with HUCPVC. (a–f) Representative immunostaining images of SK-MEL-28 tumor xenograft NOD SCID mouse model three weeks after animals were treated with two FTMs (FTM 1 and FTM 2), one term HUCPVC lines or HBSS as a control. Quantification and proportion of (a and b) Ki67+ cells (scale bar = 125 µm), (c and d) cleaved caspase-3+ cells (CC3, scale bar = 275 µm), and (e and f) isolectin B4+ cells (IB4, scale bar = 275 µm) were measured to assess proliferation, apoptosis, and vascularization, respectively. Error bars represent SEM , , , . Insets represent twofold magnification.

(a)

(b)

(c)

(d)

(e)

(f)