Human Umbilical Cord Perivascular Cells Prevent Tumor Growth in a Melanoma Tumor-Bearing Mouse Model and Modulate Breast Cancer and Melanoma Cells in a Cell Line-Dependent Manner In Vitro

Figure 4

Analysis of tumor growth, immune cells in the tumor, and serum levels of proinflammatory cytokine TNF-α and CRP, in a SK-MEL-28 tumor xenograft NOD SCID mouse model treated with FTM HUCPVC or fibroblasts. (a) Calculated tumor volume over 21 days in SK-MEL-28 tumor xenograft NOD SCID mouse model treated with one line of HUCPVC (FTM 2), fibroblast cells or HBSS (control), n = 5 per group. Animals were randomized on Day 0. Caliper measurements were done by the same blinded observer throughout the study. (b) tumor weights after dissection at 3 weeks. Error bars represent standard deviations , ; , , , . (c–g) Flow cytometric analysis showing the proportion of live cells (c), CD45+cells (d), putative NK cell (e), monocyte (f), and neutrophil (g) subpopulations. (h) Serum TNF-α levels at 5 timepoints (prestudy, Day 0, Day 7, Day 14, and Day 21). (i) Serum CRP levels in animals prestudy (n = 6) and in controls, FTM 2- and FIBS-treated animals at 3 weeks. Error bars represent standard deviations , ; , ; , ; , and . n = 5 per group for all assessments.

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