Human Placental Mesenchymal Stem Cells-Exosomes Alleviate Endothelial Barrier Dysfunction via Cytoskeletal Remodeling through hsa-miR-148a-3p/ROCK1 Pathway

Figure 3

The effects of HPMSCs-Exo on tube formation and migration capabilities of HPVECs. (a) The representative images of tube formation capability of HPVECs. (d) The average length from a randomly selected region was quantified (n > 3). The results showed that HPMSCs-Exo reversed the decline of tube formation capability of LPS-treated HPVECs (scale bar = 100 μm). (b) The representative Giemsa staining images of transwell assay of HPVECs. (e) The average number of migrated cells from a randomly selected region was counted (n > 3). The results showed that HPMSCs-Exo reversed the decrease of migration capability of LPS-treated HPVECs (scale bar = 100 μm). (c) The representative images of the migration of HPVECs, with lines approximating the cell front. (f) The average quantification of the mobility ((0−24 hr)/24 hr% area) from a randomly selected region was calculated (n > 3). The results showed that HPMSCs-Exo could improve the migration capability of LPS-insulted HPVECs. vs. NC, ^& vs. LPS.

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