Human Placental Mesenchymal Stem Cells-Exosomes Alleviate Endothelial Barrier Dysfunction via Cytoskeletal Remodeling through hsa-miR-148a-3p/ROCK1 Pathway

Figure 5

Results of RNA-seq of HPVECs. (a) KEEG pathway analysis of the DEGs in the HPVECs. The top 20 pathways with the most reliable significant enrichment were selected. Each circle represents a KEGG path; the ordinate representing the path name and the abscissa is the enrichment factor representing the proportion of genes in a differential gene annotated to a pathway to all genes. The circle color q value is the corrected P-value; the size of the circle indicates the number of genes enriched in the pathway. The path was represented by the graph near the bottom right corner (the larger the reference value). (b) The miRNA expression levels of HPMSCs-Exo (top 30). (c) The proportion of hsa-miR-148a-3p in HPMSCs-Exo miRNA profiles. (d) ROCK1 gene is the downstream target gene of hsa-miR-148a-3p. (e) Luciferase reporter assays proved that hsa-miR-148a-3p can bind to h-ROCK1-3UTR directly. . (f) HPMSCs-Exo can increase the expression level of hsa-miR-148a-3p in the LPS-insulted HPVECs. .

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