Human Placental Mesenchymal Stem Cells-Exosomes Alleviate Endothelial Barrier Dysfunction via Cytoskeletal Remodeling through hsa-miR-148a-3p/ROCK1 Pathway

Figure 6

The effects of mimic-HPMSCs-Exo on the function of HPVECs. (a and b) Overexpression of hsa-miR-148a-3p in both HPMSCs and HPMSCs-Exo was determined by q-PCR. vs. NC. (c) Representative Giemsa staining and tube-forming and migration capacity image of mimic-HPMSCs-Exo acting on HPVECs (scale bar = 100 μm). (f, g, and h) The number of migrating cells in randomly selected regions (n > 3). The results showed that mimic-HPMSCs-Exo had significantly promoted the migration of HPVECs. The mean length quantification of statistically randomly selected regions (n > 3). The results showed that mimic-HPMSCs-Exo had the strongest ability to promote angiogenesis of HPVECs. The average mobility from randomly selected regions was quantified (n > 3). The results showed that HPMSCs-Exo had the strongest ability to promote wound healing of HPVECs. vs. LPS, ^& vs. NC-mimic-HPMSCs-Exo. (d) Phalloidin staining of cytoskeleton of HPVECs (scale bar = 20 μm). (i) HPMSCs seeded on transwell plates were acted with mimic-HPMSCs-Exo, and FITC-dextran relative fluorescence intensity was determined (n > 3). vs. LPS, ^& vs. NC-mimic-HPMSCs-Exo. (e) Immunoblot analysis of ROCK1 and F-actin expression level in HPVECs. (j, k, and l) Quantification of the immunoblots, in which the results were expressed as the mean ± SD of at least three experiments. vs. NC, ^# vs. LPS, ^& vs. HPMSCs-Exo.

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