Human Placental Mesenchymal Stem Cells-Exosomes Alleviate Endothelial Barrier Dysfunction via Cytoskeletal Remodeling through hsa-miR-148a-3p/ROCK1 Pathway

Figure 7

hsa-miR-148a-3p promoted HPVECs repair via targeting ROCK1. (a) Immunoblot analysis of the overexpression of ROCK1 in HPVECs. (b) Quantification of the immunoblots, in which the results were expressed as the mean ± SD of at least three experiments. vs. NC. (c) Immunoblot analysis of HPVECs overexpressing ROCK1 by HPMSCs-Exo intervention. (d and e) Quantification of the immunoblots, in which the results were expressed as the mean ± SD of at least three experiments. vs. NC, ^# vs. LPS, ^& vs. HPMSCs-Exo + LPS. (f) FITC-dextran relative fluorescence intensity was determined of HPVECs overexpressing ROCK1 by HPMSCs-Exo intervention, in which the results were expressed as the mean ± SD of at least three experiments. vs. NC, ^# vs. LPS, ^& vs. HPMSCs-Exo+LPS. (g) Immunoblot analysis of HPVECs overexpressing ROCK1 by mimic-HPMSCs-Exo intervention. (h and i) Quantification of the immunoblots, in which the results were expressed as the mean ± SD of at least three experiments. vs. LPS, ^# vs. LPS, ^& vs. mimic-HPMSCs-Exo+LPS. (j) FITC-dextran relative fluorescence intensity was determined of HPVECs overexpressing ROCK1 by mimic-HPMSCs-Exo intervention, in which the results were expressed as the mean ± SD of at least three experiments. vs. LPS, ^# vs. LPS, ^& vs. mimic-HPMSCs-Exo + LPS.

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