HMGB1 Modulates High Glucose-Induced Erroneous Differentiation of Tendon Stem/Progenitor Cells through RAGE/β-Catenin Pathway

Figure 2

Increased expression and secretion of HMGB1 in TSPCs under high glucose. (a) Relative mRNA levels of HMGB1 in TSPCs by qRT-PCR. (b, c) Western blot analysis of HMGB1 protein expression in TSPCs. (d–f) Western blot analysis of HMGB1 protein levels in the nucleus and cytosol of TSPCs. (g) Immunofluorescence staining for HMGB1 in TSPCs. Scale bar: 50 μm. (h) ELISA analysis of HMGB1 levels in the medium supernatant of TSPCs under normal glucose or high glucose.

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