HMGB1 Modulates High Glucose-Induced Erroneous Differentiation of Tendon Stem/Progenitor Cells through RAGE/β-Catenin Pathway

Figure 7

Inhibition of β-catenin signaling mitigates high glucose-induced erroneous differentiation of TSPCs. (a) Representative images of ARS staining of TSPCs cultured with the β-catenin inhibitor PNU-74654 (50 μM) during osteogenic induction under high glucose. (b) Quantification of ARS bound to the calcium nodules in TSPCs cultured with PNU-74654. (c–e) Relative mRNA levels of osteogenic genes in TSPCs cultured with PNU-74654 during osteogenic induction by qRT-PCR. (f–h) Relative mRNA levels of tendon-related genes in TSPCs cultured with PNU-74654 by qRT-PCR. A–E × 1; F–J × 40. .

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