Effect of Doxycycline on Survival in Abdominal Aortic Aneurysms in a Mouse Model

Figure 2

Example of in vivo molecular MRI with an albumin-binding probe for the visualization of controls and aortic aneurysms in an animal model. Images from control and 4-week animals (A–J) are unpublished images from a previous study [21]. # indicates the vessel lumen, indicates the thrombus area (both nonenhancing organized thrombus and not fully organized thrombus), and § indicates an imaging artifact incomplete. A corresponds to control aortic tissue stained with elastin. B shows aortic tissue from the same probe stained with hematoxylin and eosin (H&E). C and the corresponding magnification image C+ correspond to albumin-specific staining with small immunopositive fluorescent areas of albumin. D is delayed-enhancement imaging following the administration of the albumin-binding probe gadofosveset with minimal signal enhancement. E demonstrates an aortic aneurysm after four weeks of angiotensin II infusion using elastin staining. F is a section from the same probe, stained with H&E. G and the magnification image G+ correspond to albumin-specific staining with confluent immunopositive fluorescent areas of albumin, indicating an extracellular accumulation of albumin. H is delayed-enhancement imaging following the administration of the albumin-binding probe gadofosveset with strong signal enhancement of the AAA vessel wall/thrombus area. I corresponds to an aortic aneurysm after four weeks of angiotensin II infusion and parallel treatment with doxycycline, stained with elastin. J demonstrates an aortic aneurysm with H&E staining. K and K+ correspond to the albumin-specific staining with confluent immunopositive fluorescent areas of albumin, indicating an extracellular accumulation of albumin. L is delayed-enhancement imaging following the administration of the albumin-binding probe gadofosveset with intermediate signal enhancement.