Inhibitory Effect of Natural Anti-Inflammatory Compounds on Cytokines Released by Chronic Venous Disease Patient-Derived Endothelial Cells
Figure 4
Multiplex analysis of molecular pathways activated in CVD-VEC in response to TNF-α stimulation: modulatory effect of α-Lipoic acid Ginkgoselect phytosome. Endothelial cells were left untreated (Untr.) or treated with TNF-α (5 ng/mL) alone (a) or in combination with α-Lipoic acid and Ginkgoselect phytosome (preincubation for 1 hour with α-Lipoic acid and Ginkgoselect phytosome both at 100 μg/mL before addition of TNF-α; (b)) before analysis of phosphoprotein levels performed by Luminex system and expressed as mean fluorescent intensity (MFI). In (a), endothelial cells were treated with TNF-α for the indicated time points and cell lysates were assayed for phosphoproteins activation. compared to untreated cells. In (b), VEC cultures were pretreated for 1 hour with α-Lipoic acid and Ginkgoselect phytosome prior to stimulation with TNF-α for the indicated time points. Cell lysates were assayed for phosphoproteins induction and results expressed as means ± SD of samples assayed in duplicate in four independent experiments. compared to cells treated with TNF-α alone.
