Differential Influence of Inositol Hexaphosphate on the Expression of Genes Encoding TGF-β Isoforms and Their Receptors in Intestinal Epithelial Cells Stimulated with Proinflammatory Agents

Table 1

Characteristics of primers used in experiment.

Gene	Primer sequence	Product amplified (bp)	TM (°C)
TGF-𝛽1	F: 5′-TGAACCGGCCTTTCCTGCTTCTCATG-3′	151	85
	R: 5′-GCGGAAGTCAATGTACAGCTGCCGC-3′
TGF-𝛽2	F: 5′-TACTACGCCAAGGAGGTTTACAAA-3′	201	80
	R: 5′-TTGTTCAGGCACTCTGGCTTT-3′
TGF-𝛽3	F: 5′-CTGGATTGTGGTTCCATGCA-3′	121	81
	R: 5′-TCCCCGAATGCCTCACAT-3′
T𝛽RI	F: 5′-ACTGGCAGCTGTCATTGCTGGACCAG-3′	201	81
	R: 5′-CTGAGCCAGAACCTGACGTTGTCATATCA-3′
T𝛽RII	F: 5′-GGCTCAACCACCAGGGCATCCAGAT-3′	139	84
	R: 5′-CTCCCCGAGAGCCTGTCCAGATGCT-3′
T𝛽RIII	F: 5′-ACCGTGATGGGCATTGCGTTTGCA-3′	173	85
	R: 5′-GTGCTCTGCGTGCTGCCGATGCTGT-3′

bp: base pair; TM: temperature of melting.