Proteinase-Activated Receptor-1 and Immunomodulatory Effects of a PAR1-Activating Peptide in a Mouse Model of Prostatitis

Figure 5

Instillation of the PAR1 activating peptide TFLLR-NH₂ diminishes ethanol- + dinitrobenzene sulfonic acid-induced prostatitis in wild-type mice. Prostates were treated with HEPES vehicle (HEPES), ethanol + dinitrobenzene sulfonic acid + PAR1 reverse peptide RLLFT-NH₂ (EtOH + DNBS + PAR1 RL), or ethanol + dinitrobenzene sulfonic acid + PAR1 activating peptide TFLLR-NH₂ (EtOH + DNBS + PAR-1 TF) for 2 days. The inflammatory response was measured by monitoring (a) macroscopic prostate damage, (b) microscopic prostate damage, (c) percent prostate weight, and (d) myeloperoxidase (MPO) activity. Data are mean ± SEM of 𝑛=5-6 per group. *𝑃<0.05, **𝑃<0.01, and ***𝑃<0.001 for EtOH + DNBS + PAR1 RL versus HEPES; ^#𝑃<0.05, ^###𝑃<0.001 for EtOH + DNBS + PAR1 RL versus EtOH + DNBS + PAR1 TF.

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