The Influence of Ouabain on Human Dendritic Cells Maturation

Figure 6

IL-12 secretion during DC activation. After the differentiation period (5 days), cells were incubated for further 48 h with or without TNF-α (a) (50 ng/mL) or with TNF-α in the presence or absence of 100 nM Oua (b). Then, the supernatants were collected and assayed for IL-12p40 production by ELISA. Data are expressed as cytokine concentrations (pg/mL) from six distinct individuals. ∗ and ∗∗ are significantly different from the respective controls ((a): without TNF, (b): without Oua) (^∗; ^∗∗).

(a)

(b)