The Influence of Ouabain on Human Dendritic Cells Maturation

Table 1

Viability of dendritic cells incubated with Ouabain during activation with TNF-α.

	Ctrl	Ouabain; 100 nM	Ouabain; 1 nM	Ouabain; 10 pM
DCs	0.719 ± 0.118	0.729 ± 0.155	0.738 ± 0.159	0.764 ± 0.196
DCs + TNF-α	0.852 ± 0.184	0.797 ± 0.150	0.820 ± 0.166	0.801 ± 0.222

Cells were incubated with medium alone or with 50 ng/mL TNF-α for 48 h in the presence or absence of 100 nM, 1 nM, or 10 pM Oua. The viability was analyzed by MTT assay, where values refer to the means ± SD of 3 independent experiments.