Suppressive Effects of GSS on Lipopolysaccharide-Induced Endothelial Cell Injury and ALI via TNF-α and IL-6

Figure 4

GSS inhibits LPS-induced TNF-α and IL-6 expression in ECs. After stimulation of LPS for 8 h, the endothelial cells were collected, and the mRNA and protein expressions of IL-6 and TNF-α were detected by RT-PCR and ELISA. (a) ECs were pretreated with GSS for 2 h and then exposed to LPS, and the expression of IL-6 was detected by RT-PCR. (b) The PCR result was presented as a histogram showing the band intensity values. (c) The RT-qPCR results of IL-6 were presented as a histogram. The relative amount was normalized to the housekeeping gene GAPDH. (d) ECs were pretreated with GSS for 2 h and then exposed to LPS, and the expression of TNF-α was detected by RT-PCR. (e) The PCR result was presented as a histogram showing the band intensity values. (f) The RT-qPCR results of TNF-α were presented as a histogram. The relative amount was normalized to the housekeeping gene GAPDH. (g) ECs were pretreated with GSS for 2 h and then stimulated with LPS; the level of IL-6 in supernatant of ECs was determined by ELISA analysis. (h) ECs were pretreated with GSS for 2 h and then stimulated with LPS; the level of TNF-α in the supernatant of ECs was determined by ELISA analysis. versus negative control. ^# versus the corresponding LPS treatment group.

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