The Severity of CVB3-Induced Myocarditis Can Be Improved by Blocking the Orchestration of NLRP3 and Th17 in Balb/c Mice

Figure 6

Blockade of IL-1β can significantly alleviate the severity of VMC in Balb/c. (a) Statistical analysis of survival rate from day zero to day sixteen in control, anti-IL-1β, saline, and ISO groups. (b) HE images of myocardial in control, anti-IL-1β, saline, and ISO groups at sixteenth day of CVB3-induced VMC process (original magnification ×100). (c) Masson-trichrome staining of myocardial in control, saline, ISO, and anti-IL-1β groups at sixteenth day of CVB3-induced VMC process (original magnification ×100). (d) IHC images of IL-17 in myocardial in control, saline, ISO, and anti-IL-1β groups at sixteenth day of CVB3-induced VMC process (original magnification ×100). (e) IL-17 expression in heart tissue acquired by IHC assay and IOD value was calculated by Image-Pro Plus. (f, g) The relative mRNA expression of RORγt and IL-17 in myocardium in control, anti-IL-1β, saline, and ISO groups at sixteenth day of CVB3-induced VMC process. (h) The protein level of IL-17 in plasma in control, anti-IL-1β, saline, and ISO groups at sixteenth day of CVB3-induced VMC process. (i) The proportion of Th17 cells in spleen samples in control, anti-IL-1β, saline, and ISO groups at sixteenth day of CVB3-induced VMC process. (j) Statistical histogram of Th17 proportion in (i). Statistical description: ns, , , and stand for no statistically significant difference, , , and , respectively, for anti-IL-1β group versus saline or ISO group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)