Basil Polysaccharide Reverses Development of Experimental Model of Sepsis-Induced Secondary Staphylococcus aureus Pneumonia

Figure 2

CLP led to damaged pulmonary immune responses in the host. Mice receiving CLP or sham operation. (a, b) Histological scores for CLP-induced nonsevere sepsis model ( mice/group). (c, d) At 24 h and 72 h after CLP, we detected contents of cytokines in serum and BALF. Mice Cytokine Magnetic Bead Panel Kit ( for every group) was performed to analyze the obtained specimens. (e) 72 h after CLP, mice were given intratracheal injection with S. aureus ( CFU). Following challenge ( for every group), we observed mortality rates over the 10-day period. Log-rank (Mantel-Cox) test was performed to analyze survival curves. (f) At 24 h following secondary infection with S. aureus, we detected contents of chemokines and cytokines in serum and BALF. Mice Cytokine Magnetic Bead Panel Kit ( for every group) was performed to analyze the obtained specimens. Values were presented in the manner of , whereas nonparametric Mann–Whitney test was adopted for data analysis. , , compared with normal control or sham-surgery mice upon secondary S. aureus infection.

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