Basil Polysaccharide Reverses Development of Experimental Model of Sepsis-Induced Secondary Staphylococcus aureus Pneumonia

Figure 3

Postseptic basil polysaccharide is resistant to S. aureus pneumonia. (a) Survival of mice treated with basil polysaccharide upon S. aureus infection during sepsis ( mice/group). (b) Typical HE staining for lung tissue samples at 24 h postinfection with S. aureus during sepsis and following treatment with IgG or basil polysaccharide group. (c) Histological scores for secondary pulmonary infection with S. aureus within septic mice, as well as following treatment with IgG or basil polysaccharide ( mice/group). (d, e) TUNEL assay was performed to determine cell apoptosis, where the nuclei of TUNEL-positive cells were dark-brown. (f) BALF and serum cytokine or chemokine contents were detected at 24 h after treatment with IgG or basil polysaccharide during sepsis-induced secondary S. aureus pneumonia in mice. Specimens were collected for analysis by Mice Cytokine Magnetic Bead Panel Kit ( mice/group). Survival curves were analyzed using the log-rank (Mantel-Cox) test. Data were expressed as , whereas nonparametric Mann–Whitney test was applied for data analysis. , , and , relative to secondary pulmonary infection with S. aureus of septic mice in the IgG group.

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