Basil Polysaccharide Reverses Development of Experimental Model of Sepsis-Induced Secondary Staphylococcus aureus Pneumonia

Figure 5

Effect of recombinant protein CCL4 (CC receptor ligand 4) on resistance in septic mice with S. aureus pneumonia. Recombinant protein CCL4 was administered (500 ng) 2 hours after S. aureus inoculation in septic mice. The control group was given equivalent IgG control. (a) Survival of septic mice with secondary S. aureus infection ( mice/group) following recombinant protein CCL4 administration. (b, c) Blood and lung CFU of septic mice with secondary S. aureus infection ( mice/group) following recombinant protein CCL4 administration. (d–f) Lung damage assessment indicators such as protein in BALF, myeloperoxidase (MPO), and wet/dry weight ratio in septic mice with secondary S. aureus infection ( mice/group) following recombinant protein CCL4 administration. (g) The total number of macrophages in BALF in septic mice with secondary S. aureus infection ( mice/group) following recombinant protein CCL4 administration. (h, i) Histological scores for secondary pulmonary infection with S. aureus of septic mice ( for every group). Log-rank (Mantel-Cox) test was performed to analyze survival curves. Values were presented in the manner of , whereas nonparametric Mann–Whitney test was adopted for data analysis. ^#, , , and , relative to secondary pulmonary infection with S. aureus of septic mice receiving recombinant protein CCL4 treatment. (j) The concentration of CCL4 in the cell supernatant after S. aureus or basil polysaccharide stimulates neutrophils for 48 hours. , , upon one-way ANOVA and LSD multiple comparisons, relative to the S. aureus group.

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