Involvement of Dendritic Cells and Th17 Cells in Induced Tertiary Lymphoid Structures in a Chronic Beryllium Disease Mouse Model

Figure 3

Characterization of inflammatory structures in the CBD lungs. Immunohistochemistry of lung sections, together with hematoxylin and eosin (H&E) staining. (a) Limited numbers of CD11c⁺ cells in the lungs of PBS control mice. (b) Abundant presence of CD11c⁺ cells forming granuloma-like structures in CBD mice 16 weeks after initial exposure Be BeO (c) Gr-1⁺ granulocytic inflammation in CBD mice 8 weeks after initial exposure. (d) Diffuse and dense infiltration of CD4⁺ (red) and CD8⁺ (blue) T cells. (e) Perivascular focal dense infiltration, reflecting TLS, consisting of B220⁺ B cells (blue) and CD3⁺ T cells (red) in the lungs of CBD mice at 8 weeks. Data are representative for 5-7 mice analysed in each group. Bars are 20 μm (d), 50 μm (a, b, e), or 100 μm (c).

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