Involvement of Dendritic Cells and Th17 Cells in Induced Tertiary Lymphoid Structures in a Chronic Beryllium Disease Mouse Model

Figure 4

DC subset analysis in BAL fluid of CBD mice. (a) Quantification of DC subset numbers in BAL of PBS-treated mice (white bars) and CBD mice (black bars) 8 weeks after initial exposure. (b) Pie charts showing proportions of DC subsets in BAL fluid of PBS-treated and CBD mice. (c) DC activation as measured by flow cytometry staining of surface expression of MHCII and CD40 on gated CD11c⁺ cells from the nonautofluorescent fraction. (d) Quantification of the indicated macrophage and monocyte fractions in BAL of PBS-treated mice (white bars) and CBD mice (black bars) (steady-state (S.s.) monocyte; inflammatory (Infl.) monocytes). Pie charts showing proportions of macrophage/monocytes in BAL fluid of PBS-treated and CBD mice. Data are expressed as the (a, d) (Mann-Whitney test: ). Data are from mice per group and representative for 2 experiments.

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