Involvement of Dendritic Cells and Th17 Cells in Induced Tertiary Lymphoid Structures in a Chronic Beryllium Disease Mouse Model

Figure 8

IL-17 signaling is important for TLS formation in CBD pathology. (a) Immunohistochemical analysis of lung tissue sections stained for B220⁺ B cells (blue) and CD3⁺ T cells (red) of the lungs obtained from CBD WT and CBD IL-17R KO mice, as indicated, at 8 weeks after Be/BeO exposure. Arrows indicate perivascular focal dense infiltrates, reflecting TLSs of the lungs of CBD mice. Scale bars: 500 μm (upper panels) and 50 μm (lower panels). (b) Quantification of focal dense infiltrates in lung sections of the indicated mice. (c) Immunohistochemical analysis of a lung tissue section for IgD (red) and the GC marker GL7 (blue) from a WT CBD mouse, conforming the presence of a GC in the TLS 20 μm. (d) Quantification of flow cytometric analyses of the indicated B-lineage populations of single-cell lung digest (d). The results shown are expressed as the and represent one out of two independent experiments with 4-7 mice per group. Mann-Whitney test: , , and .

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