An Aptamer-Based Antagonist against the Receptor for Advanced Glycation End-Products (RAGE) Blocks Development of Colorectal Cancer

Figure 2

Inhibitory effect of Apt-RAGE on S100B-induced synthesis and secretion of VEGF-A protein. (a) HCT116 cells were starved and pretreated with 100 nM Apt-RAGE or Ctrl-Apt for 90 min in the presence of S100B (2 μg/mL). Phosphorylation of NFκB, VEGF-A, and RAGE was examined using western blotting. (b) ELISA assay was used to determine VEGF-A release in the culture medium treated with S100B (2 μg/mL) in the presence or absence of Apt-RAGE (100 nM) or Ctrl-Apt (100 nM). Data are presented as the , vs. untreated control and in Apt-RAGE vs. S100B. (c) Apt-RAGE inhibited S100B-independent phosphorylation of NFκB in RAGE-overexpressed cells. HCT116 cells transfected with pcDNA3.1-RAGE or pcDNA3.1 for 48 h and incubation with Apt-RAGE (100 nM) for 20 min. Phosphorylation of NFκB and the expression of VEGF-A and RAGE were examined using western blotting.

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