Extracellular HMGB1 Induced Glomerular Endothelial Cell Injury via TLR4/MyD88 Signaling Pathway in Lupus Nephritis

Figure 3

HRGEC injury contributed to the upregulation of glomerular filtration membrane permeability in LN. (a) TEER of HRGECs stimulated by LN plasma at different time was measured (). (b) HRGEC glomerular filtration membrane permeability was assessed by the FITC-BSA method (). (c) Change in TEER and glomerular filtration membrane permeability of HRGEC. (d) IF staining showing the expression of SDC-1 (green) in HRGECs. (e) The level of SDC-1 in supernatant was measured by ELISA (). (f) Colocalization of VE-cadherin (green) and F-actin (red) in HRGECs was determined by IF staining. (g) Western blotting of VCAM-1 in LN plasma-stimulated HRGECs. (h) The level of NO in HRGECs was measured (). vs. control group. TEER: transendothelial electrical resistance.

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