Smurf1 Facilitates Oxidative Stress and Fibrosis of Ligamentum Flavum by Promoting Nrf2 Ubiquitination and Degradation

Figure 4

Smurf1 facilitated the fibrosis and oxidative stress of LF cells. (a, b) the transfection efficiency of Smurf1 was analyzed by qRT-PCR and western-blot. . (c, d) Western blotting and semiquantification for Collagen I, Collagen III, and α-SMA mRNA levels in the LF cells with or without Smuf1-OE). . . (e) The ROS levels and MDA content was assessed in the LF cells with or without Smuf1-OE. . . (f) the Glutathione (GSH) content, and Superoxide dismutase (SOD) activity was assessed in the LF cells with or without Smuf1-OE. . .

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