IgE Immune Complexes Mitigate Eosinophilic Immune Responses through NLRC4 Inflammasome

Figure 1

Ova-IgE ICs upregulated NLRC4 inflammasome components in TLR2-primed EoL-1 cells. EoL-1 human eosinophils were primed with TLR2 ligand PAM3CSK4 (1 µg/ml) and treated with IgE (10 µg)-Ova (1 µg) ICs. (a) NLRC4 mRNA expressions by real-time qPCR after Ova-IgE ICs treatment in TLR2-primed EoL-1 human eosinophils. Values represent the mean ± SD and are representative of three separate experiments. Student’s t-test shows the significant difference between treated and nontreated cells. (b and c) Western blot and corresponding densitometry analyses of cell lysates (30 µg). NLRC4, caspase-1, and IL-1β protein expressions were immunoblotted. Values represent the mean ± SD and are representative of three separate experiments.

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