Salvianolic Acid B Alleviates High Glucose-Induced Vascular Smooth Muscle Cell Inflammation by Upregulating the miR-486a-5p Expression

Figure 2

Sal B reduces HG-induced VSMC inflammation. (a, b) VSMCs were incubated with LG (5.5 mM), HG (25 mM), and HG (25 mM) plus different concentrations of Sal B (2.5, 5, and 10 μM) for 24 hr. ELISA detected the IL-1β and TNF-α concentrations of the culture medium. and vs. HG group (n = 5). (c) VSMCs were stimulated as in 2(a). Relative expression of IL-1β and TNF-α mRNA was examined by qRT-PCR and presented after normalizing to GAPDH (means ± SEM, n = 3). and vs. HG group. (d) VSMCs were incubated as in 2(a). The protein expression of IL-1β and TNF-α was detected by western blotting (left panel). Bar graphs show the relative level of these proteins, which were repeated three times. and vs. HG group (right panel, n = 3). (e, f) The VSMCs were cultured with LG (5.5 mM), HG (25 mM), and HG (25 mM) plus Sal B (10 μM) for 6, 12, and 24 hr. ELISA measured the IL-1β and TNF-α concentrations of the culture medium. and vs. HG group (n = 5). (g, h) The VSMCs were stimulated as in 2(e). Relative expression of IL-1β and TNF-α mRNA was examined by qRT-PCR and presented after normalizing to GAPDH (means ± SEM, n = 3). The protein expression of IL-1β and TNF-α was detected by western blotting (left panel). , , and vs. HG group n = 3.

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