Salvianolic Acid B Alleviates High Glucose-Induced Vascular Smooth Muscle Cell Inflammation by Upregulating the miR-486a-5p Expression

Figure 3

Sal B inhibits HG-induced VSMC inflammation by downregulating FOXO1. (a–d) The VSMCs were treated as in 2(a) or 2(e). qRT-PCR and western blot analysis measured the expression of FOXO1. , , and vs. HG group (n = 3). (e, f) The FOXO1 expression in the mouse aortic vessels was measured by qRT-PCR and western blot analysis. vs. DM group (n = 7). (g, h) The VSMCs were transfected with si-FOXO1 or si-control for 24 hr and then incubated with HG (25 mM) plus or without Sal B (10 μM). ELISA measured the IL-1β and TNF-α concentrations in the culture medium. vs. each control group (n = 5). (i, j) The VSMCs were treated as in 3(g), and qRT-PCR and western blot analysis measured the expression of IL-1β and TNF-α in VSMC. vs. each control group (n = 3). (k, l) VSMCs were transfected with pcDNA3.1 or pcDNA3.1-FOXO1 for 24 hr and then treated with HG (25 mM) plus or without Sal B (10 μM). The expression levels of IL-1β and TNF-α were detected by ELISA. and vs. each control group (n = 5). (m, n) The VSMCs were treated as in 3(k). The expression levels of IL-1β, TNF-α, and FOXO1 were measured by qRT-PCR and western blot analysis. and vs. each control group (n = 3).

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