Salvianolic Acid B Alleviates High Glucose-Induced Vascular Smooth Muscle Cell Inflammation by Upregulating the miR-486a-5p Expression

Figure 4

Sal B downregulates HG-induced VSMC inflammation by upregulating miR-486a-5p. (a) The VSMCs were treated with LG (5.5 mM) or HG (25 mM) for 24 hr, and qRT-PCR detected the miRNA expression. and vs. LG group (n = 3). (b, c) The VSMCs were treated as in 2(a) or 2(e). qRT-PCR measured the miR-486a-5p expression. and vs. HG group (n = 3). (d) qRT-PCR measured the miR-486a-5p expression in the mouse aortic vessels. vs. DM group (n = 7). (e, f) The VSMCs were transfected with mimic-control or miR-486a-5p mimic for 24 hr and then treated with HG (25 mM) plus or without Sal B (10 μM). ELISA measured the IL-1β and TNF-α concentrations of the culture medium. and vs. each control group (n = 5). (g, h) The VSMCs were cultured as in 4(e). qRT-PCR and western blot analysis measured the IL-1β and TNF-α expression. , , and vs. each control group (n = 3).

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