Salvianolic Acid B Alleviates High Glucose-Induced Vascular Smooth Muscle Cell Inflammation by Upregulating the miR-486a-5p Expression

Figure 5

miR-486a-5p targets FOXO1. (a) miR-486a-5p binding site in the 3′-UTR of FOXO1 mRNA (green). Blue is the mutation position. (b, c) Luciferase reporter assays in 293A cells were cotransfected with the constructs containing wt or mutant (mut) FOXO1 3′-UTR and miR-486a-5p mimic/inhibitor or mimic-control/inhibitor-control. Experiments were performed in triplicate and each value is the mean ± SEM of three independent experiments. vs. mimic-control or inhibitor-control group (n = 3). (d, e) VSMCs were transfected with indicated constructs for 24 hr, and qRT-PCR or western blot detected FOXO1 expression (left panel). vs. mimic-control or inhibitor-control group (n = 3).

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