Research Article
Qingfei Tongluo Mixture Attenuates Bleomycin-Induced Pulmonary Inflammation and Fibrosis through mTOR-Dependent Autophagy in Rats
Figure 4
QFTL attenuates bleomycin-induced fibroblast activation. (a) Western blot detection shows the expression of pulmonary fibrosis-related proteins α-SMA and MMP12. Results are expressed as relative ratios of band density of α-SMA (b) and MMP12 (c). (d) Immunohistochemical staining of α-SMA was performed for further validation. Representative photomicrographs of immunohistochemical staining in lung tissues from different treatment groups. (e) Quantitative evaluations were performed by mage-Pro Plus 6.0 software to calculate the positive area proportion of α-SMA. α-SMA, α-smooth muscle actin; MMP12, matrix metalloproteinase 12; data presented are means ± SD. vs. control; # vs. model; Δ vs. PFD.
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