Research Article
Tanshinone IIA Alleviates Traumatic Brain Injury by Reducing Ischemia‒Reperfusion via the miR-124-5p/FoxO1 Axis
Figure 4
Tanshinone IIA attenuates the I/R-induced inflammatory response and neuronal apoptosis by upregulating miR-124-5p. (a) RT-qPCR was used to analyze the mRNA expression of miR-9, miR-124-5p, miR-132 and miR-148a-3p; (b) the transfection efficiency of miR-124-5p was analyzed by RT-qPCR; (c–e) ELISA analysis of cytokine expression; (f, g) flow cytometric analysis of apoptosis; (h) the expressions of apoptosis-related proteins were analyzed by western blot; (i) RT-qPCR was used to analyze the mRNA expression of apoptosis related proteins. , compared with the NC group; #, ## compared with the OGD group.
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