Cryptotanshinone Inhibits Bladder Cancer Cell Malignant Progression in a Lipopolysaccharide-Induced Inflammatory Microenvironment through NLRP3 Inhibition

Figure 2

Lipopolysaccharide (LPS) upregulated NLRP3 and inflammatory cytokine expression in bladder cancer cells. In total, 5,637 cells were grown and treated with LPS and/or MCC950 and then subjected to (a) RT-qPCR analysis of NLRP3 mRNA and (b and c) Western blot analysis of NLRP3 protein. The cell culture supernatant was gathered and subjected to enzyme-linked immunosorbent assays (ELISAs) to determine the levels of (d) IL-1β, (e) IL-18, and (f) TNF-α. , , , and .

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