Cryptotanshinone Inhibits Bladder Cancer Cell Malignant Progression in a Lipopolysaccharide-Induced Inflammatory Microenvironment through NLRP3 Inhibition

Figure 6

NLRP3 overexpression reversed cryptotanshinone (CTS)-reduced lipopolysaccharide (LPS)-induced inflammatory cytokine expressions in bladder cancer cells. In total, 5,637 cells were grown and pretreated with LPS, then transfected with pcDNA3.1-NLRP3 or vector-only as a negative control, and finally treated with CTS before being subjected to (a) RT-qPCR analysis of NLRP3 mRNA and (b and c) Western blot analysis of NLRP3 protein. The cell culture supernatant was gathered and subjected to enzyme-linked immunosorbent assays (ELISAs) to determine the levels of (d) IL-1β, (e) IL-18, and (f) TNF-α. NC, negative control; OE, overexpression. , , , and .

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